The physical layer defines the electrical and physical specifications of the data connection. It defines the relationship between a device and a physical transmission medium (for example, an electrical cable, an optical fiber cable, or a radio frequency link). This includes the layout of pins, voltages, line impedance, cable specifications, signal timing and similar characteristics for connected devices and frequency (5 GHz or 2.4 GHz etc.) for wireless devices. It is responsible for transmission and reception of unstructured raw data in a physical medium. Bit rate control is done at the physical layer. It may define transmission mode as simplex, half duplex, and full duplex. It defines the network topology as bus, mesh, or ring being some of the most common.

The physical layer is the layer of low-level networking equipment, such as some hubs, cabling, and repeaters. The physical layer is never concerned with protocols or other such higher-layer items. Examples of hardware in this layer are network adapters, repeaters, network hubs, modems, and fiber media converters.